Isaeva G.A., Kiselev K.V.
В журнале Journal of Biotechnology
Год: 2010 Том: 150 Номер: Supp. 1 Страницы: 481
Resveratrol displays a wide range of biological and pharmacological properties (Aggarwal et al., 2004 B.B. Aggarwal, A. Bhardwaj, R.S. Aggarwal, N.P. Seeram, S. Shishodia and Y. Takada, Role of resveratrol in prevention and therapy of cancer: preclinical and clinical studies, Anticancer Research 24 (2004), pp. 2783–2840. View Record in Scopus | Cited By in Scopus (340)Aggarwal et al., 2004). A better understanding of the regulation of resveratrol biosynthesis has fundamental and applied interests. We investigated the effects of salycilic acid (SA) treatment and transformation with rol genes of Agrobacterium rhizogenes on the levels of resveratrol production and stilbene synthase gene expression in cell cultures of Vitis amurensis. Transformation of V. amurensis V2 callus culture by the rolB gene of Agrobacterium rhizogenes resulted in more than a 100-fold increase in resveratrol production in transformed calli. The rolB-transformed calli are capable of producing up to 3.2% dry wt. of resveratrol. In the rolB transgenic cultures expression of six STS genes was increased, while expression of VaSTS6 was not significantly changed. The SA-treated calli are capable of producing up to 0.1% dry wt. of resveratrol (Kiselev et al., 2010). SA increases expression of certain genes of VaSTS family: VaSTS2, VaSTS3, VaSTS4, VaSTS5, VaSTS6, and VaSTS8. We compared the data concerning STS gene expression in SA-treated calli with the results previously obtained on rolB transgenic cultures of V. amurensis with high levels of resveratrol production. The effects of SA treatment and rolB transformation on VaSTS gene expression considerably differ. These results suggest that rolB and SA increases resveratrol production via selective enhancement of expression of individual genes from STS gene family.
