Koren O.G., Gorpenchenko T.Yu., Muzarok T.I., Zhuravlev Yu.N.
В издании International Symposium for the Sustainable Forest Ginseng Production, November 9-10
Год: 2007 Страницы: 83-97
Panax ginseng is a rare plant of Far Eastern forests and well-known herb of the Oriental traditional medicine. To protect ginseng resources from exhaustion, a long-term program of reintroduction of Russian ginseng population was designed. In this work, some genetic peculiarities of wild-growing ginseng were evaluated using allozyme markers. For investigation on genetic structure of the natural ginseng populations, allele frequencies were studied in eight sub-populations and the main parameters of genetic variation of wild-growing P. ginseng were determined. Overall differentiation test revealed significant differences in sub-population allele frequencies. Some lack of variation was observed in the Sikhote-Alin population of P. ginseng. It was shown, that the highest values of expected heterozygosity were found in the sub-populations of the Blue Mountain and the Khasan populations. To investigate mating system of P. ginseng, the gene segregations within seed families of ginseng plants were researched with allozyme markers. Parental genotypes were determined and segregations of the genotypes within all seed families were studied for three polymorphic allozyme loci. Tests on autogamy, outcrossing and agamospermy yielded seed sets 53.5%, 45.3% and 9.4%, respectively. Analysis of allele segregations within seed families of P. ginseng showed that hand self-pollination yielded a higher seed set than cross-pollination and agamospermy. Results of the agamospermy experiment revealed genotype auto-segregation corresponding to a disomic gamete auto-segregation. It was suggested that agamospermy is a reserve mechanism of ginseng reproduction. Basing on these results some practical reccomendations have proposed.
