Spiridonova L.N., Korobitsyna K.V., Yakimenko L.V.
В издании Modern achievements in population, evolutionary and ecological genetics (MAPEEG – 2007): Program and Abstract of International Symposium
Год: 2007 Страницы: 37
The practicability of mtDNA markers application for the diagnostic purposes depends on as far as character of microevolutionary transformations of nuclear and mitochondrial genomes coincides in this or that group of organisms (Sheldon et al., 2000). Theoretically, in an ideal at concurrence of variability regularities, any nuclear genome of each taxon should correspond to its own unique mtDNA haplotype. However actually it is not always observed and in the nature there is the variety of both genomes. The aim of the present work is the analysis and comparison of subspecific markers distribution of different inheritance systems (nDNA (RAPD) and mtDNA) in some localities of Mus musculus. 94 wild mice were used as a material from 14 localities of Russia from Novosibirsk region to Kamchatka and Primorye. Individuals of the following subspecies: musculus, castaneus, domesticus and gansuensis typified by morphology, karyotype and mtDNA were served as the subspecies standard. The investigation of different genetic markers (nDNA and mtDNA) has revealed discrepancy of geographic distribution of nuclear and mitochondrial subspecific attributes, although the same specimens were used as a source. The strict correlation between the investigated markers is absent in the majority of localities. In the same time the increased variety of nuclear sequences accompanied by prevalence of one of mtDNA haplotype is marked. According to data of H. Yonekawa (Yonekawa et al., 2003) received by sequencing of high polymorphic region in mtDNA D-loop (266 bp) and our results received also of mtDNA data, but full-size sequence of D-loop (880 bp), musculus hyplotype was extended on the most part of the Asian continent. Castaneus haplotype was marked only in the north of the Far East. Nuclear markers of musculus, domesticus, castaneus and gansuensis were present at the investigated samples in different ratios, and in the majority of cases features of nominative subspecies M. musculus prevailed. Nuclear characteristic of domesticus with different frequency are revealed in many localities analyzed. On the contrary, RAPD-markers of subspecies castaneus and gansuensis are not met to the west from Baikal like. Taxon-specific diversity of house mice nDNA in each locality considerably exceeds mtDNA variety in which musculus haplotype tends to dominate. The RAPD-analysis of house mice has found out that the same individual can carry nDNA fragment which marks different M. musculus subspecies. Such a pattern specifying on mixture of nuclear attributes of different subspecies at the same individual confirms a hybrid origin of house mice fauna on the territory observed. It reflects a real situation in geographical distribution of taxonomic genetic markers involved in hybridization.
