Odintsova N., Kiselev K., Sanina N., Kostetsky E.
В журнале Cryoletters
Год: 2001 Том: 22 Номер: 5 Страницы: 299-310
Primary cell cultures obtained from somatic and larval tissues of bivalve molluscs and from embryos of sea urchins were frozen to -196 degreesC by two-step freezing using 10% dimethyl sulfoxide (DMSO) or/and trehalose (3-30mg/ml) as cryoprotectants. We estimated both cell viability and the RNA synthetic activity after freeze-thaw, Total lipid extracts from the tissues of echinoderms examined as possible cryoprotective agents demonstrated a weak cryoprotective capacity. Mussel lipid extract was found to possess a considerable cryoprotective activity. Cryoprotective capacity of tested lipids correlated with their thermotropic behaviour, DMSO + trehalose combination was shown to be a favourable cryoprotectant and sea urchin blastula cells the most freezing-tolerant cells.
