Aleynova O.A., Dubrovina A.S., Suprun A.R., Ogneva Z.V., Kiselev K.V.
В журнале Plant Cell, Tissue and Organ Culture
Год: 2024 Том: 159 ArticleID: 77
Alternative splicing (AS) is a non-canonical gene splicing process that allows a single gene to produce multiple protein isoforms and increase the diversity of protein functions. This study investigates the involvement of AS in plant tolerance to abiotic stresses using the calcium-dependent protein kinase (VaCPK21) gene involved in the tolerance to salinity in grapevine Vitis amurensis Rupr. In this study, we investigated expression of VaCPK21 under the effect of various environmental stresses, and, in addition to the full-length VaCPK21, we obtained two short spliced transcripts and one longer transcript. While recombinant VaCPK21 enhanced salt stress resistance, overexpression of the short VaCPK21s1 and VaCPK21s2 transcripts in grapevine cell cultures and Arabidopsis did not confer tolerance to the tested stresses. Overexpression of the longer VaCPK21L1 did not confer tolerance to salt stress but conferred a novel tolerance to osmotic stress induced by mannitol. Overexpression of all VaCPK21 transcripts did not affect the biosynthesis of stilbenes, valuable phenolic secondary metabolites. Thus, the VaCPK21s1 and VaCPK21s2 isoforms appear to lose the stress tolerance properties of the full-length VaCPK21, while the VaCPK21L1 isoform confers novel tolerance to osmotic stress. These findings show that AS can result in the loss of properties characteristic of the original canonical form or the appearance of new properties, which is important for full understanding of CPK and alternative splicing biological functions.
