Guro P.V., Sazanova A.L., Kuznetsova I.G., Tikhomirova N.Y., Belimov A.A., Yakubov V.V., Safronova V.I.
В журнале Russian Journal of Plant Physiology
Год: 2023 Том: 70 Страницы: 185
A collection of twenty-eight strains isolated from root nodules of Astragalus inopinatus Boriss. and Astragalus umbellatus Bunge originating from the Kamchatka Peninsula (Russian Federation) was obtained for the first time. Analysis of the 16S ribosomal RNA gene sequence revealed a significant diversity of isolates belonging to 4 genera of the order Hyphomicrobiales: Mesorhizobium, Rhizobium, Bosea and Tardiphaga. The presence of phenotypically and taxonomically different strains (Bosea + Tardiphaga, Bosea + Mesorhizobium, Tardiphaga + Mesorhizobium, Bosea + Rhizobium) in some nodules of both plants was also observed. The symbiotic efficiency of strains isolated from A. inopinatus plants in the nodulation assay showed that most isolates of the genus Mesorhizobium and Rhizobium can form a nitrogen-fixing symbiosis with A. inopinatus, leading to a significant increase in plant biomass compared to the non-inoculated control.
