Aleynova O.A., Nityagovsky N.N., Suprun A.R., Kiselev K.V.
В журнале Applied Biochemistry and Microbiology
Год: 2022 Том: 58
A new approach based on endophyte products is proposed to enhance the biosynthesis of stilbenes (compounds with established health benefits) and to alter their qualitative profile in grape-cell cultures. For this, 13 products are developed based on bacterial and fungal endophytes from the wild-grown grape species Vitis amurensis Rupr. With the use of bacterial and fungal endophyte based-products, the stilbene content increased by a factor of 1.3-1.5 and 2.0-3.5, respectively. The highest increase in the stilbene content (up to 3.07 mg/g on a dry cell biomass bases) is observed in grape cells cultured for 3 days in the presence of an endophyte product based on a Trichoderma sp. (10 mg). It is established that the increase in the stilbene content observed in V. amurensis grape cells cultured in the presence of endophyte products is due to a significant upregulation of genes responsible for stilbene biosynthesis, i.e., phenylalanine ammonia lyase (PAL) and stilbene synthase (STS). Products based on native endophytes from V. amurensis are attractive and environmentally friendly stimulators for stilbene biosynthesis in grape-cell cultures.
