Malinovskii V.I., Seletskaya L.D., Varfolomeeva L.A., Kuguk N.S.
В журнале Russian Journal of Plant Physiology
Год: 1993 Том: 40 Номер: 5 Страницы: 785-790
The viability of protoplasts and cells isolated from leaves of sensitive (cv. Samsun) and hypersensitive (cv. Xanthi nc) tobacco plants and incubated in either liquid or agar nutrient medium after infection with tobacco mosaic virus (TMV) was studied. No decrease in the protoplast and cell viability was observed in samples obtained from infected Samsun plants. Protoplasts and cells isolated from Xanthi nc plants during the appearance of necrosis had low viability. Xanthi nc protoplasts remained viable if they were infected after the isolation, or if the infected plants were cultivated at 32-degrees-C. Though the cells obtained from these plants were viable immediately after isolation, the fraction of the dead cells increased further during incubation. The induction of the N gene expression in the isolated cells was inhibited by actinomycin D. The viability of the cells and protoplasts incubated in various nutrient media did not differ significantly. It is supposed that the cell wall presence was necessary for the N gene induction and necrosis development.
