Wan X., Jin X., Wu X., Yang X., Lin D., Li C., Fu Y., Liu Y., Liu X., Lv J., Gontcharov A.A., Yang H., Wang Qi, Li Yu
В журнале Carbohydrate Polymers
Год: 2022 Том: 276 ArticleID: 118798
The medicinal fungus Sanghuangporus vaninii can be cultivated in large scale and has outstanding antitumour activity. In this study, water-soluble S. vaninii polysaccharides (SVPs) were extracted from fruiting bodies. Four polysaccharide sub-fractions (SVP-W, SVP-1, SVP-2 and SVP-3) were isolated, with molecular weights from 90.50 kDa to 261.70 kDa, and all inhibited the proliferation of non-small cell lung cancer cell lines A549, 95-D and NCI-H460, especially the acidic SVP-1. SVP-1 affected cell morphology and colony formation in NCI-H460 cells. It also promoted cell apoptosis following nuclear fluorescence staining and flow cytometry. Methylation and nuclear magnetic resonance analyses revealed that SVP-1 is a heteroglycan with the main chain →4)-β-DGlcp-( 1 → 6)-β-D-Glcp-(1 → 6)-α-D-Galp-(1 → 6)-β-D-Glcp-(1→, and the branched chain α-D-Manp-(1 → 2)-α-DManp-( 1 → 3)-β-D-Glcp-(1 → 3,6)-β-D-Glcp-(1→. The findings indicate that this natural acidic polysaccharide has potential for non-small cell lung cancer therapy.
