Gafitskaya I.V., Orlovskaya I.Yu., Nakonechnaya O.V., Nesterova S.V.
В журнале Botanica Pacifica: a journal of plant science and conservation
Год: 2020 Том: 9 Номер: 1 Страницы: 85 - 90
Microclonal propagation of Dasiphora fruticosa Duham. (wild form (Df0) and cultivars 'Lovely Pink' (Df1), 'Bella Bianca' (Df2), 'Bella Sol' (Df3) was investigated in details. Microplants were cultured on hormone-free solid 1/2 Murashige and Skoog medium. Well-formed plants with developed root system were established after 2 months of culturing. The maximum values of shoot height, number of internodes, and root mass were observed in the Df1 microplants, with a greater number of roots in the Df0 microplants. The use of half-strength medium for both growth initiation and microplant propagation allows saving reagents and reducing the cost of D. fruticosa microclonal multiplication in vitro. The use of ready-mixed soil with ph 5.6-6.7, and cultural vessels for growth of microplants under controlled conditions such as temperature, light and humidity made it possible to successfully adapt more than 90 % of test-tube microplants to the soil conditions.
