Tarbeeva D.V., Fedoreyev S.A., Veselova M.V., Blagodatski A.S., Klimenko A.M., Kalinovskiy A.I., Grigorchuk V.P., Berdyshev D.V., Gorovoy P.G.
В журнале Fitoterapia
Год: 2019 Том: 135 Страницы: 64-72
Four new pterocarpans (6aR,11aR)-6a,11a-dihydrolespedezol A 2 (2), (6aR,11aR)-2-isoprenyl-6a,11a-dihydrolespedezol A 2 (3), (6aR,11aR,3′R)-6a,11a-dihydrolespedezol A 3 (4), (6aR,11aR,3′S)-6a,11a-dihydrolespedezol A 3 (5) and one new stilbenoid with 1,2-diketone fragment named bicoloketone (6) along with one previously known pterocarpen lespedezol A 2 (1) have been isolated from Lespedeza bicolor stem bark using multistage column chromatography on polyamide and silica gel. The structures of the isolated polyphenolic compounds were determined by spectroscopic methods. The absolute configurations of 4 and 5 were determined by comparison of their electronic circular dichroism (ECD) spectra obtained experimentally and the spectra calculated using time-dependent density functional theory (TDDFT). The isolated compounds exhibited a moderate DPPH scavenging effect and ferric reducing power compared to the reference antioxidant quercetin. The cytotoxicity of compounds against three human cancer cell lines, HTB-19, Kyse-30, and HEPG-2, and two normal cell lines, RPE-1 and HEK-293, was tested using the MTT assay. Compound 3 showed the strongest cytotoxic activity against all cell lines (IC 50 6.0–19.1 μM) compared with the positive control cisplatin. The other tested compounds possessed moderate cytotoxic activity against cancer cells.
