Kozyrenko M. M., Artyukova E. V., Lauve L. S., Boltenkov E. V.
В журнале Биотехнология
Год: 2002 Номер: 4 Страницы: 30–37
Six species of Iris L. genus (I. setosa, I. ensata, I. oxypetala, I. pseudocorus, I. pumila, and I. laevigata) were comparatively studied using the RAPD and cytogenetic analyses of genomes of their callus cultures and intact donor plants. The closest genetic similarity was observed in callus cultures derived from the same donor plant and the least similarity, in calli from the plants of different genera. During culturing, genetic changes occur on interspecies and intraspecies levels, and the extent of these changes seems to depend on species. A cytogenetic analysis of callus cultures and donor plants of the six Iris species showed that the modal class of cell population is constituted by the cells with the diploid chromosome set of parent donor plant. Hence, the in vitro cell populations retain the basic genome features of the species despite their significant genetic heterogeneity.
