Rapd-pcr analysis of genetic variability of two anser species (A.albifrons and A.fabalis)

Volkovsky D.V., Zhuravlev Yu.N.

В издании Modern achievements in population, evolutionary and ecological genetics (MAPEEG - 2009): Program and Abstract of International Symposium

Год: 2009 Страницы: 50

RAPD-PCR analysis of 54 samples of Anser albifrons (Scopoli, 1769) and 28 samples of Anser fabalis (Latham, 1787) collected during their migration through Primorsky Krai (Russian Far East) was carried out. Eight primers (5'>TCG GCG ATA G<3', 5'>GTG AGG CGT C<3', 5'>GAT GAC CGC C<3', 5'>TGG ACC GGT G<3', 5'>TGT CAT CCC C<3', 5'>CAC ACT CCA G<3', 5'>TGA GCG GAC A<3', 5'>ACG GTA CCA G<3') were used to estimate basic genetic parameters for these species. It was shown that interspecific genetic distances varied from 0,0603 to 0,1148. The intraspecific genetic distances in A. albifrons ranged from 0,0214 to 0,0645, and in A. fabalis - from 0,02 to 0,0648. Polymorphism value (P95) of A. albifrons was equal 14,64%, and that of A. fabalis was 20,32%. The average index of population subdivision (Gst) was equal 0,503. The expected heterozygosity of A. albifrons and A. fabalis were 0,0596 – and 0,0872, respectively.

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