Koldaev V., Manyakhin A., Zorikova O., Zorikov P., Belov A.
В журнале Research Journal of Chemistry and Environment
Год: 2017 Том: 21 Номер: 5 Страницы: 35-40
The study is dedicated to absorption spectrophotometry of herbal formulations. The objective is to develop the methodology for determining numerical indicators of the turning points, steps and maximums of the absorption spectrum (AS) lines to evaluate the quality of herbal extractions. Ultraviolet absorption spectrophotometry of the extractions was prepared in different conditions from garden sage leaves and hawthorn berries. AS have been processed with proprietary software calculating the wavelengths, optical densities, first derivatives in the turning points, steps, maximums and rates of the absorption bands. For the purposes of statistical processing Student's ttest and Z-Sign test have been applied. When the conditions for preparing the extractions were changed, the variations of values of the derivatives in the turning points and steps proved to be most evident and the changes of values of optical densities and wavelengths in AS maximums were less expressed. Significant differences from the standard AS numerical indicators were observed in cases when the extractions were prepared under varying conditions or with substandard raw material quality giving grounds for rejecting the extracts under investigation. Due to the sufficient pharmaceutical precision, low associated costs and the possibility to obtain the results quickly, it seems advisable that the numerical indicators under investigation should be used for the purpose of spectrophotometric fast analysis in the course of evaluating the quality and authenticity of herbal formulations. The aggregate of the obtained numerical indicators represents a spectrophotometric passport of the extraction and can be included in the regulatory documents for pharmaceutical drugs of plant origin. Numerical indicators of absorption spectra in herbal formulation quality assessment. Available from: https://www.researchgate.net/publication/317164265_Numerical_indicators_of_absorption_spectra_in_herbal_formulation_quality_assessment [accessed Dec 14 2017].
