Zhuravlev Y.N., Yudakova Z.S., Pisetskaya N.F.
В журнале Virology
Год: 1976 Том: 73 Номер: 2 Страницы: 454-460
Tobacco cv. Samsun protoplasts were inoculated with TMV in the presence of different polyelectrolytes. Retention of [14C]TMV was determined by radioactivity of the protoplasts or by infectivity in homogenates of samples frozen immediately after inoculation and washing. Virus yield was determined by infectivity in protoplasts following incubation for 48 hr in light at 28°. Infectious TMV, noninfectious TMV, and TMV protein (100 μg/ml) enhanced virus retention. However, the virus yield for the first substance was essentially lower. When the substances were added to the incubation medium, they decreased virus yield. Bovine serum albumin and casein hydrolysate did not appreciably affect retention; yet, they also decreased virus yield. Virus RNA, whether infective or not, strongly suppressed the virus yield when present in the inoculation medium at a concentration of 1 μg/ml or more. This effect was independent of the presence of actinomycin D in the medium. Experiments with labeled TMV revealed the low virus yield to be the result of a reduction of virus retention in inoculum containing a mixture of TMV and TMV RNA. Similar effects on virus retention and yield were produced by total RNA of animal origin and by dextran sulfate. The authors assume that the three above-mentioned polyelectrolytes block virus adsorption on the plasmalemma by binding with a virus-polyornithine complex. © 1976.
