Park I-S, Kim T-M., Hur J.W., Kartavtseva I.V.
В журнале Korean Journal of Genetics
Год: 2005 Том: 27 Номер: 2 Страницы: 141-144
We report a simple method involving the injection of a active yeast to increase the number of mitotic cells in the bone marrow tissue of the soft-shelled turtle, Pelodiscus sinensis Crother. Intramuscular injections of a suspension of active yeast into the hind leg region elevated the bone marrow mitotic index by as much as 10-fold. The metaphases induced using this method showed excellent chromosome spreading when air-dried. This method should prove useful to investigators who wish to use bone marrow as a source of chromosomes for soft-shelled turtle cytogenetic studies
