Aleynova O.A., Dubrovina A.S., Kiselev K.V.
В журнале Магарач. Виноградарство и виноделие
Год: 2015 Том: 4 Страницы: 9-11
Stilbenes are acting as phytoalexins and possess valuable biologically active properties. Stilbenes are synthesized via the phenylpropanoid pathway, where stilbene synthase (STS) condenses three molecules of malonyl-CoA and one molecule of cumaryl-CoA to form resveratrol. Resveratrol may be metabolized to form other stilbenes as viniferins, pterostilbene, and piceid. In grape genome STS exist as a multigene family yielded at least 32 potentially functional genes per haploid genome. All STS differ on amino-acid structure; perhaps it affects different ability to synthesize the resveratrol. We obtained several independently transformed Vitis amurensis cell lines, overexpressed different grape STS genes: three cell lines, overexpressed VaSTS1 gene; two VaSTS2-transgene cell lines; and three VaSTS7-overexpressing cell lines. Overexpression of all VaSTS gene increased total stilbenes content and production (mainly trans-resveratrol and t-resveratrol glycosides) compared with control calli transformed empty vector, but only in the one VaSTS2- and one VaSTS7-transgene cell lines this increasing was significant. Using p-coumaric acid (CA), a precursor of stilbenes and isoflavonoids, we stimulated stilbenes content and production in all VaSTS-transgene cell lines. The highest stimulated effect was detected in VaSTS2- and VaSTS7-transgene cell lines: after CA feeding stilbenes content and production increased in 1.5-2.4 times. Total stilbenes content enhanced through increasing in the t-resveratrol and t-resveratrol glycosides content. Thus, we demonstrated that VaSTS2 and VaSTS7 showed more stimulated effect on the stilbenes accumulation than VaSTS1. The data suggest that VaSTS2 and VaSTS7 genes had more activity in the stilbenes biosynthesis.
