Kiselev K.V., Aleynova O.A., Dubrovina A.S.
В журнале Магарач. Виноградарство и виноделие
Год: 2015 Том: 3 Страницы: 43-45
Resveratrol is a naturally occurring plant stilbene that exhibits a wide range of valuable biological and pharmacological properties. Although the beneficial effects of trans-resveratrol to human health and plant protection against fungal pathogens are well-established, yet little is known about the molecular mechanisms regulating stilbene biosynthesis in plant cells. It has been recently shown that calcium ionophore A23187 increased resveratrol levels, while the calcium channel blockers and an antagonist of calcium-dependent protein kinases (CDPKs or CPKs), W7 (N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide), significantly reduced the accumulation of resveratrol in cell cultures of wild grapevine Vitis amurensis Rupr. In plants, CDPKs are currently considered as the key calcium sensor proteins in calcium-mediated signaling. In the V. amurensis genome, CDPKs exist as a multigene family consisting of at least 13 potentially functional genes per haploid genome. In our work, we overexpressed 6 VaCPK genes (VaCPK3a, VaCPK9, VaCPK13, VaCPK20, VaCPK21, and VaCPK29) in callus cell cultures of V. amurensis. Our data indicate that the VaCPK20 and -29 genes are implicated in resveratrol biosynthesis in V. amurensis as positive regulators, while the VaCPK3a, -9, -13, and -21 genes are not involved in the regulation of stilbene biosynthesis in the grape cells.
