Kamenskaya D.N., Pankova M.V., Atopkin D.M., Brykov V.A.
В журнале Molecular Biology
Год: 2015 Том: 49 Номер: 5 Страницы: 687-693
The growth-hormone gene is presented as à single copy in the genome of most vertebrates. In contrast, in salmonids, one of the genome duplication events resulted in the multiplication of many genes, including the growth hormone. In salmonids, the growth-hormone gene exists as two independently inherited functional paralogues, gh1 and gh2. In this study, we performed a comparative analysis of gh1 and gh2 growth-hormone genes and their adjacent sequences in Levanidov’s charr Salvelinus levanidovi to determine their functionality and define the potential differences. We found that both genes have the same gene structure and are composed of six exons (I–VI) and five introns (A, B, C, D, E). However, the respective gene sequences differ in length. A comparison of exons showed that the size of each exon is identical in both paralogues. The overall length of genes differs due to the varying lengths of introns. Coding sequence of both genes contains an open reading frame for 210 amino acids. We identified regulatory elements in the promoter region of both genes: TATA box, A/T-rich regions that contain binding sites for pituitary-specific transcriptional activator Pit-1, and regions responsible for interaction with other transcriptional activators and initiators, in particular hormone receptors. Thus, the obtained data indicates that both gh1 and gh2 genes in Levanidov’s charr Salvelinus levanidovi genome are functional.
