Jung S. W., Vshivkova T. S., Bae Y. J.
В журнале Canadian Entomologist
Год: 2016 Том: 148 Номер: 2 Страницы: 123-139
Mitochondrial DNA (mtDNA) sequences which serve as DNA barcodes have been used to associate immature and adult stages of insects and to delineate species. The partial mitochondrial cytochrome c oxidase subunit I (COI) gene sequences for Korean Megaloptera (all known in the adult stage) were tested to identify undetermined larvae as a rapid and effective method from 31 specimens (16 adults and 15 larvae). The COI gene sequences distinguished all six known Megaloptera species, with a low genetic distance between larvae and adults (0.50±0.21%). Based on the COI gene sequences, we associated five types of larvae with known adults including the following four species of newly described larval stages: Sialis annae Vshivkova 1979, Neochauliodes formosanus (Okamoto 1910), Parachauliodes asahinai Liu, Hayashi et Yang 2008, and Protohermes xanthodes Navas 1913. The known larval stage of Sialis koreana Jung et Bae 2012 was confirmed, and the morphological variation in the male genitalia of Sialis longidens Klingstedt 1932 is discussed. A larval key to the six Korean species of Megaloptera is provided.
