The influence of the rolC gene on isoflavonoid production in callus cultures of Maackia amurensis

Grishchenko O.V., Kiselev K.V., Tchernoded G.K., Fedoreyev S.A., Veselova M.V., Bulgakov V.P., Zhuravlev Y.N.

В журнале Plant Cell, Tissue and Organ Culture

Год: 2013 Том: 113 Номер: 3 Страницы: 429-435

The polyphenolic complex of Maackia amurensis, as well as a complex of isoflavonoids from M. amurensis callus cultures, display strong hepatoprotective effects in experimental animal and human studies. To increase the yield of polyphenols in cultures of M. amurensis, calli were transformed with the rolC gene or with an empty vector as a control. HPLC analysis revealed that the transgenic cultures produced the same complex of isoflavonoids. The complex consisted of 20 compounds, including isoflavones and their glucosides as well as pterocarpans and their glucosides. The cultures transformed with either the empty vector or the rolC gene construct produced on average 1.22% DW and 1.39% DW of isoflavonoids, respectively. Isoflavonoid production in the transformed callus lines carrying the empty vector and the rolC gene construct reached 106 mg/L and 146 mg/L, respectively. Moreover, the rolC gene construct promoted cell growth and overall cell productivity. The transgenic callus lines expressing the rolC gene exhibited higher levels of the following six isoflavonoids: daidzein, calycosin, formononetin, 4'-?-?-glucopyranosyldaidzin, maackiain and 6'-O-malonyl-3-O-?-D-glucopyranosylmaackiain. However, lower levels of genistin were observed in rolC calli than in those carrying the empty vector.

DOI 10.1007/s11240-012-0283-x