Analysis of the genetic structure of Rhodiola rosea (Crassulaceae) using inter-simple sequence repeat (ISSR) polymorphisms

Kozyrenko M. M., Gontcharova S. B., Gontcharov A. A.

В журнале Flora: Morphology, Distribution, Functional Ecology of Plants

Год: 2011 Том: 206 Номер: 8 Страницы: 691-696

Genetic diversity and differentiation of eleven R. rosea populations from different parts of its wide areal was studied by ISSR markers. Using eight primers, 252 DNA fragments were generated with 243 DNA fragments being polymorphic, indicating a high genetic variability at the species level (P = 96.4%, h = 0.176, SI = 0.291). The relatively low levels of diversity were determined at the population level (P 30.6–59.1%, h 0.088–0.165, SI 0.137–0.257). AMOVA revealed that the most part of genetic variation was within populations (65.42%) and variance among populations was 34.58%. Cluster analysis revealed two groups of R. rosea populations. These groups likely represent distinct evolutionary lines in the species, which are different in genetic structure, evolutionary history and migration routes.

DOI 10.1016/j.flora.2010.12.002

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