Vainutis K. S., Voronova A. N., Maximov R. A., Bogatov V. V.
Journal of Ichthyology 2026; 66(3): 353-365
We present the first molecular-genetic analyses of Acheilognathus chankaensis free embryos flood- plain lake of the Razdolnaya River basin (Primorsky region, Russia). Examination of 115 Nodularia dougla- siae individuals revealed 24 infested bivalves hosting 219 free embryos (length 3–5 mm). Genetic analysis of 23 specimens via cox1 mtDNA sequencing confirmed identity with A. chankaensis populations from North- east China and South Korea. Phylogenetic reconstruction (Bayesian inference, maximum parsimony, max- imum likelihood) of six Acheilognathinae genera resolved nine generic groups and two distinct lineages of A. chankaensis: sensu stricto (11 haplotypes in haplogroups A1 (Primorye−Korea−Heilongjiang) and A2 (Yangtze basin)) and a polyphyletic A. chankaensis sensu lato group. Our results demonstrate possible post- glacial dispersal patterns via connected drainages (haplogroup A1), ancient vicariance in South China (hap- logroup A2), and taxonomic discordance requiring revision of A. chankaensis s.l.
