FIALKO A.V., YUGAI Y.A., VASYUTKINA E.A., GRIGORCHUK V.P., SHKRYL Y.N., BULGAKOV V.P.
Доклады Российской академии наук. Науки о жизни 2026; 527: 135–143
The work investigated the effect of repeated cold stress on physiological, transcriptional and metabolic changes in Arabidopsis thaliana. The experiment included stages of primary cooling, acclimatization and re-exposure to cold. Repeated cold stress caused increased electrolyte leakage and a decrease in chlorophyll a content, indicating disruption of membrane integrity and restructuring of the photosynthetic apparatus. Expression analysis revealed increased activity of the DREB1A, MYC2, and COR15A and COR15B genes, which are associated with the cold response and the formation of stress memory. Concurrently, transcription of the flowering regulators FLC, HDA6, and HD2C was altered, ensuring a balance between adaptation and development. HPLC-MS analysis revealed significant accumulation of flavonoids, primarily kaempferol and quercetin derivatives, indicating activation of antioxidant defenses. Taken together, these data confirm that repeated cold exposure activates a complex plant adaptation system based on the integration of physiological, transcriptional, and metabolic mechanisms. The formation of stress memory allows A. thaliana to maintain resistance and functional stability during repeated low-temperature exposures.
