VASYUTKINA E.A., YUGAY Y.A., KUDINOVA O.D., SHKRYL Y.N., BULGAKOV V.P.
Доклады Российской академии наук. Науки о жизни 2026; 529: 489-495
Naturally integrated rol-like genes of sweet potato (Ipomoea batatas (L.) Lam.) are of interest as potential regulators of physiological and biochemical processes in plants. In this study, we evaluated the effects of the Ib-rolB/C gene on the growth and metabolic characteristics of callus cultures of the model plant Arabidopsis thaliana. Gene expression had no significant effect on biomass accumulation but was accompanied by an almost twofold increase in total phenolic content. The transgenic lines also exhibited enhanced antiradical activity and reduced malondialdehyde levels, indicating strengthened antioxidant protection and decreased lipid peroxidation. Expression analysis revealed elevated transcript levels of PAL, PBS3, LOX2, AOC1, OPR2, and OPR3, suggesting activation of the phenylpropanoid pathway and salicylic acid- and jasmonic acid-related signaling pathways. These findings indicate that Ib-rolB/C is involved in regulating phenolic metabolism and the defensive status of cultured plant cells.
