Gorpenchenko Tatiana Y., Marina L. Sidorenko, Valeria P. Grigorchuk,· Evgeniya S. Martynenko, Polina A. Perepelkina, Maria T. Khandy, Alexandra V. Kim, Vitalii M. Kazarin, Vyacheslav V. Makhankov, Yuriy N. Zhuravlev
Horticulture, Environment, and Biotechnology 2026
(BC) containing three microorganism strains (nitrogen-fixing strain Acinetobacter johnsonii A1; potassium-solubilizing strain Pseudarthrobacter equi S2; and phosphate-solubilizing strain Pantoea agglomerans F19) was used to promote Panax ginseng C.A. Meyer growth and productivity. Morphometric, microbiological and metagenomic analyses revealed that the BC treatment had a positive effect on the growth and development of ginseng plants in artificial fields and increased the biogenicity of the soil, leading to the development of rare bacterial and fungal species. Compared with that in the control plots, the number of fungal cells in the treated rhizosphere decreased by half. HPLC‒MS analysis revealed changes in the composition of ginseng root metabolites and a 1.5-fold increase in the content of ginsenosides. Importantly, rare polar dammarane glycosides from the protopanaxadiol group were identified in the roots of the BC-treated plants. Among these, ginsenosides Ra1, Ra2, and Ra3, which contain five monosaccharide residues, were present at the highest concentration of 3.26 mg/g dry weight (DW), accounting for 11% of the total ginsenosides. These polar Ra-group ginsenosides have previously been characterized as possessing anticancer activity.
