Tarasova A.A., Ponomarenko M.G.
A.I.Kurentsov memorial lectures 2022; (33): 55–68
An analysis of intraspecific variability based on the barcoding fragment mtCOI showed that species of Y. padella -complex with larger ranges are characterized by relatively larger intervals of genetic distances between representatives of different populations compared to species distributed in a limited area. Thus, for the Transpalaearctic species Y. evonymella , the intraspecific distance lies within 0-1%, while the average indices of intraspecific distances were revealed in the Euro-American Y. malinella and the European Y. irrorella (0-0,5%), and the minimal range of distances were recorded in the Euro-American Y cagnagella and European Y. rorrella (0-0,2%). For the East Asian Y. orientalis , in which samples were studied only from the Russian Far East, intraspecific variability was not revealed. The interspecific distance intervals within the Y. padella -complex turned out to be very low and with small range, not exceeding 2,6% ( Y. padella - Y. orientalis , Y. padella - Y. irrorella ) and 2,8% ( Y. malinella - Y. irrorella , Y. malinella - Y. orientalis ), which confirmed the weak divergence of the species group not only in morphological markers, but also in the barcoding fragment mtCOI. In a number of studied species, the intervals of intraspecific and interspecific distances have borderline indicators or slightly overlapping ones - 0,2% ( Y. cagnagella ), 0,2-0,3% ( Y. padella ), 0,5-0,7% ( Y. malinella ), which point out the unreliability of fragment COI usage to identify such weakly divergent species as the species of the Y. padella -complex. Summarizing the above, it should be concluded that identification of a number of taxa, especially relatively young and weakly divergent ones, is difficult based on the nucleotide sequences of the barcoding fragment mtCOI without the use of a complex of other markers.
