Kholina A.B., Kozyrenko M.M., Artyukova E.V., Andrianova E.A., Sandanov D.V., Selyutina I.Y., Khoreva M.G.
Bulletin of the North-East scientific center 2019; (2): 117-125
Based on polymorphism of the psbA-trnH, trnL-trnF, and trnS-trnG intergenic spacers of the chloroplast DNA, the genetic diversity and phylogenetic relationships of oxytropis evenorum Jurtz. et Khokhr., spread in Northeast Asia, were studied. Haplotype diversity in populations from Magadan Oblast varies from 0.356 to 0.857; nucleotide diversity, from 0.0001 to 0.0016. The population from Kamchatka is monomorphic. In general, the species is characterized by a high level of haplotype (0.909) and relatively low level of nucleotide (0.0032) diversity. Statistically significant values of genetic distances between populations vary from 0.42120 to 0.98043. Molecular dispersion analysis (AMOVA) showed that about 81% of all genetic variability is due to variability among populations (ФST = 0.80959, p < 0.0001). The reconstruction of phylogenetic relationships of closely related species confirms the idea of the hybridogenic origin of O. evenorum, involving O. ochotensis.
