Subbotin E.P., Gafitskaya I.V., Nakonechnaya O.V., Zhuravlev Yu.N., Kulchin Yu.N.
Turczaninowia 2018; 21(2): 32–39
The light source (Sun Box) was developed by the authors for the first time, and its emission spectrum was in the range of frequency 440-660 nm coincided with the solar spectrum. Its effect and light intensity on the growth and development of plants were studied. The experiments were carried out on regenerating plants of Solanum tuberosum L. ('Rozhdestvenskiy' and 'Snegir' cultivars), cultivated under in vitro conditions. Virus-free plantlets obtained by the method of the apical meristem isolation were used. A high intensity of the artificial solar spectrum (230.1 and 382 mu mol/s*m(2)) was shown to suppress plant growth, the number of leaves and the green mass of plants were decreased. But additional shoot formation was observed under high levels of irradiance (382 mu mol/s*m(2)): two or three small shoots developed on all plantlets in the group. At an intensity of 135.5 mu mol/s*m(2), a significant increase in plant height, leaf size and number of leaves was observed. Irradiance of 74.6 mu mol/s*m(2) had no effect on the height of plants and the number of leaves, while the increase in the root mass was found. The data obtained can be used to optimize the regime of cultivation of potato regenerating plants in vitro and to adapt them to open ground as well as to accelerate the growth of plants under controlled conditions, for example, in autonomous spaces of ships or in the extreme North conditions.
