Makhazen D.S.
Vestnik of the Far East Branch of the Russian academy of sciences 2017; (4): 130-134
JAZ (jasmonate ZIM-domain) proteins are negative regulators of jasmonic acid signaling pathway in plant cells which is involved in activation of secondary metabolism. We have constructed artificial microRNAs (amiRNA) that specifically inhibit expression of JAZ genes of grape (Vitis vinifera) and arabidopsis (Arabidopsis thaliana) - VvJAZ1 and AtJAZ1, respectively. These amiRNA were transferred to pPZP binary vector intended for plant cells genetic transformation. The offered approach is a new method of getting cultures - producers of valuable secondary plant metabolites by activation jasmonate-dependent signaling.
