Burenina E.A.
Russian journal of parasitology 2014; (4): 48-54
Activities and properties of 5'-nucleotidase in the subcellular fractions of Bothriocephalus scorpii are studied with the use of adenosine monophosphate, inosine monophosphate and cytidine monophosphate as substrates. The high activity of 5'-nucleotidase was observed in the mitochondrial fraction. Dependence of the 5'-nucleotidase activity on the concentration of substrates and Mn 2+ ions is investigated. Impact of various effectors and ions (sodium fluoride, EDTA, cysteine, K +, Na +, Zn 2+, Ca 2+, Ba 2+, Cu 2+, Mn 2+) on enzyme activity is determined. Effect of 10 anthelmintic drugs on enzyme activity has been tested. The most effective drugs are bitionol and oxinide.
