Pislyagin E.A., Yurchenko E.A., Gorpenchenko T.Y., Davidova V.N., Aminin D.L.
Международный журнал прикладных и фундаментальных исследований 2014; (1): 116-120
The molecular mechanism of the immunostimulatory action of triterpene glycoside cucumarioside A2-2 isolated from the Far Eastern sea cucumber Cucumaria japonica was studied. Using fluorescent imaging approach the interaction of cucumarioside A2-2 with membrane receptors of mouse peritoneal macrophages, mainly purine receptors of P2X family (as the membrane targets of glycoside action) was investigated. A pharmacological typing of different membrane receptors and ion channel by various blockers and modulators of Ca2+ transport was carried out. Using flow cytometry and FACS-analysis technique a composite structure of the mouse immune cell population and their participation in the immune response to the glycoside application was revealed.
