Burenina E.A.
Journal of Evolutionary Biochemistry and Physiology 2011; 47(3): 251-259
Activity and properties of fructose bisphosphatase (FBPase) were studied in the free-living turbellarian Phagocata sibirica. All subcellular fractions of P. sibirica (12 000 g cytosol, 105 000 g cytosol, mitochondria, and microsomes) have the FBPase activity. Dependence of the FBPase reaction rate on the substrate concentration was studied. For realization of the enzyme activity, the high affinity to substrate and the presence of bivalent cations (Mg 2+ or Mn 2+) are necessary. There was studied the effect of various effectors as well as of monovalent (Na +, K +, Li +, and NH +) and bivalent (Zn 2+ and Cu 2+) cations.
