Gulyaeva M., Sobolev I., Sharshov K., Kurskaya O., Alekseev A., Shestopalova L., Kovner A., Bi Y., Shi W., Shchelkanov M., Shestopalov A.
В журнале Virologica Sinica
Год: 2018 Том: 33 Номер: 5 Страницы: 449-452
In the present study, 27 Caspian seals of 18–20 months age (17 female and 10 male) were captured during an integrated research on AIV at Zhemchuzhny island (Astrakhan Region, Russia) in 2012. Sera samples were collected from the captured seals and tested for H1, H3, H5 and H7 speci?c antibodies. All animals were sera negative for these subtypes. Nasopharyngeal swabs were collected and tested for the presence of AIV using real-time PCR with previously published primers and probes targeting the matrix (M) gene. One PCR positive sample was collected from the eighteen months old female seal without clinical signs. The sample was inoculated into 10-day-old embryonated chicken eggs. Allantoic ?uid was collected 3 days post infection. The resulting strain belongs to the low pathogenic AIV and was named as A/Caspian seal/Russia/T1/2012(H4N6). The full-length genome of the isolate was sequenced using MiSeq (Illumina, USA) according to the manufacturer’s instructions. Phylogenetic analysis of all viral genes was performed using the MEGA v6.0 software with the maximum-likelihood method and 1000 bootstrap replications. Phylogenetic analysis showed that all genes of A(H4N6)T1 were closely related to avian-derived in?uenza viruses of the classical Eurasian lineage circulating in wild birds.