Kholina A.B., Kozyrenko M.M., Artyukova E.V., Koldaeva M.N., Sandanov D.V., Selyutina I.Yu.
В журнале Russian Journal of Genetics
Год: 2021 Том: 57 Номер: 9 Страницы: 1042–1056
On the basis of the analysis of the nucleotide polymorphism of the intergenic spacers psbA–trnH, trnL–trnF, and trnS–trnG of chloroplast DNA of the Oxytropis species from Asian Russia, O. tragacanthoides section Hystrix subgenus Tragacanthoxytropis, O. coerulea, O. filiformis, and O. mandshurica sect. Janthina, and O. deflexa and O. glabra sect. Mesogaea subg. Phacoxytropis, it was found that all populations are characterized by high haplotype diversity (h varies from 0.676 to 1.000), except for species of the sect. Mesogaea (h varies from 0 to 0.333). Species-specific markers were found for O. tragacanthoides, O. deflexa, O. glabra, and O.mandshurica, as well as specific markers for the sect. Mesogaea. Reconstruction of the phylogenetic relationships of the chlorotypes of the species of the subg. Phacoxytropis, Tragacanthoxytropis, and Oxytropis showed that the species of the sect. Janthina are combined into one well-supported clade with the species of the subg. Tragacanthoxytropis and Oxytropis, but their relationships remained unresolved. An analysis of the genealogical relationships of the ribotypes of the ITS of nuclear DNA revealed a common ribotype for the species O. tragacanthoides, O. coerulea, O. lanata, O. chankaensis, O. oxyphylla, and O. triphylla, belonging to three subgenera. The revealed genetic affinity with clear morphological differences is characteristic of taxa with a common origin that have experienced relatively recent rapid adaptive radiation. The obtained data on the variability of markers of the nuclear and chloroplast genomes confirm the status of O. coerulea, O. filiformis, and O. mandshurica as three separate species.
