- ВЫБОРЫ ДИРЕКТОРА
Tyunin A.P., Kiselev K.V.
В журнале Plant Cell, Tissue and Organ Culture
Год: 2016 Том: 124 Страницы: 33-45
Ultraviolet (UV) emerging with the sun light plays crucial role in plants ontogenesis as the one of the main environmental stresses. Prolonged exposure to UV causes damage to DNA, proteins and membranes, and the inhibition of protein synthesis and photosynthetic reactions. Moreover, UV radiance is known to induce metabolic modifications in plants, particularly to secondary metabolite biosynthesis. Multitude of the studies published to date considering exposure of grape cells to UV revealed enhancement in stilbene compounds production, and many of which possess unique biological and pharmacological properties. However, the epigenetic mechanisms regulating expression of the genes involved in stilbene biosynthesis in response to UV exposure were not studied. In this study as model objects were used grape Vitis amurensis plant cell cultures capable to produce trans-resveratrol (t-resveratrol). T-resveratrol, a naturally occurring plant phenol, has been reported to exhibit a wide range of valuable biological and pharmacological properties. T-resveratrol synthesized via the phenylpropanoid pathway, where phenylalanine ammonia-lyase (PAL) is the first enzyme in this pathway and stilbene synthase (STS) is the enzyme that directly catalyzes the reaction of t-resveratrol formation. Exposure of V. amurensis cells to UV of C type significantly increased t-resveratrol production and certain STS and PAL expression. Using bisulfite sequencing, we demonstrated that methylation level of the VaSTS2, VaSTS6 and VaSTS10 genes significantly decreased, while methylation level of constitutively expressed VaSTS1 gene did not changed significantly.